Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

Julie King, Surbhi Grewal, Manel Othmeni, Benedict Coombes, Cai-yun Yang, Nicola Walters, Stephen Ashling, Duncan Scholefield, Jack Walker, Stella Hubbart-Edwards, Anthony Hall, Ian King
Triticum timopheevii (2n=28, AAGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n=42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the A and G subgenomes of T. timopheevii. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors brought upon wheat production due to climate change.
This data is made available under the Toronto Agreement
All of the data listed here is available under the prepublication data sharing principle of the Toronto agreement. By using this data, you agree to:
  • respect the rights of the data producers and contributors to analyze and publish the first global analyses and certain other reserved analyses of this data set in a peer-reviewed publication.
  • not redistribute, release, or otherwise provide access to the data to anyone outside of the group, until the data has been published & submitted to the public data repositories.
  • contact the authors to discuss any plans to publish data or analyses that utilize this data to avoid the overlap of any planned analyses.
  • fully cite the prepublication data along with any applicable versioning details.
  • understand that this data as accessed is precompetitive and is not patentable in its present state.
This agreement does not expire by time but only upon publication of the first global analysis by the data producers and contributors.
      Search:

          User details

          You are browsing the public view on the public zone
          NameSizeDateProperties
          iRods Data ObjectR0130-S0010_timoP95_A08676_2_1_HY5JVDSXX_ACAGTG_L003_R1.fastq.gz 40GB2022-04-12 10:31
          View metadata for R0130-S0010_timoP95_A08676_2_1_HY5JVDSXX_ACAGTG_L003_R1.fastq.gz as CSVView metadata for R0130-S0010_timoP95_A08676_2_1_HY5JVDSXX_ACAGTG_L003_R1.fastq.gz as JSON
          iRods Data ObjectR0130-S0010_timoP95_A08676_2_1_HY5JVDSXX_ACAGTG_L003_R2.fastq.gz 43GB2022-04-12 10:38
          View metadata for R0130-S0010_timoP95_A08676_2_1_HY5JVDSXX_ACAGTG_L003_R2.fastq.gz as CSVView metadata for R0130-S0010_timoP95_A08676_2_1_HY5JVDSXX_ACAGTG_L003_R2.fastq.gz as JSON
          iRods Data Objectdatapackage.json3.0KB2022-04-15 14:10
          View metadata for datapackage.json as CSVView metadata for datapackage.json as JSON